![]() Furthermore, the cryo-EM reconstruction revealed an unusual conformation of the spike where two RBDs are in the ‘up’ ACE2-binding conformation. ![]() A cryo-EM structure of the spike bound to Sb23 showed that Sb23 binds competitively in the ACE2 binding site. ![]() Several binders with low nanomolar affinities and efficient neutralization activity were identified of which Sb23 displayed high affinity and neutralized pseudovirus with an IC 50 of 0.6 µg/ml. Here, we report the rapid isolation and characterization of nanobodies from a synthetic library, known as sybodies (Sb), that target the receptor-binding domain (RBD) of the SARS-CoV-2 spike protein. However, traditional antibody production is hampered by long development times and costly production. Therapeutic neutralizing antibodies constitute a key short-to-medium term approach to tackle COVID-19. The coronavirus SARS-CoV-2 is the cause of the ongoing COVID-19 pandemic. Nature Communications volume 11, Article number: 5588 ( 2020) ![]() Selection, biophysical and structural analysis of synthetic nanobodies that effectively neutralize SARS-CoV-2 ![]()
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